NUCLEIC ACID SYNTHESIS AND TURNOVER IN TISSUE CULTURE
نویسندگان
چکیده
منابع مشابه
Nucleic acid synthesis and turnover in tissue culture.
It has been demonstrated (1) that the cultivation of embryonic chick tissues in roller tubes in the presence of an extract of chick embryo resulted in an increase in the protein content of the cultures. Histological examination of the tissue revealed a progressively diminishing cellular density in the tissue following cultivation in embryo extract. This decrease in the cellular concentration of...
متن کاملProtein synthesis and amino acid turnover in tissue culture.
The tissue culture method permits the prolonged cultivation of living cells isolated from the environmental influence of the intact organism. Under optimal nutritional conditions, the tissue grows by rapid proliferation of new cells from the original explants, resulting in a net increase in the quantity of protein and nucleic acid (1). Under these conditions the cultures are comparable to syste...
متن کاملNucleic Acid Synthesis
INTRODUCTION Synthesis in the field of nucleic acids has been a major and continuing interest in my laboratory ever since we knew the structures of the nucleic acids. Clearly the foundations of synthetic work in the nucleotide field were laid by the prolonged and intensive efforts of Professor Todd's laboratory (Cambridge, England) and it was my good fortune to be associated with this laborator...
متن کاملEvaluation of Nucleic Acid Sequence Based Amplification (NASBA) and Reverse Transcription Polymerase Chain Reaction for Detection of Coxsackievirus B3 in Cell Culture and Animal Tissue Samples
Enteroviruses are the causative agents of a number of diseases in humans. Group B coxsackieviruses are believed to be the most common viral agents responsible for human heart disease. Genomic data of enteroviruses has allowed developing new molecular approaches such as Nucleic Acid Sequence Based Amplification (NASBA) for detection of such viruses. In this study, coxsackievirus B3 (CVB3) was de...
متن کاملTissue tropism of Ornithobacterium rhinotracheale in chickens determined by culture and nucleic acid detection.
The aim of the present study was to evaluate the ability of Ornithobacterium rhinotracheale (ORT) to colonize chosen organs of chicks infected intratracheally (group A1), or intravenously (group A2), with the use of bacteriological methods and PCR. The bacteriological methods enabled to reisolate ORT bacteria from trachea and lungs of the birds from group A1 only on day 3 and 6 after infection....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1952
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)55706-9